《植物生理学报》 2014, 50(9): 1336-1346

国内大豆品种资源中滞绿(stay-green)性状的初步研究

任钧¹, 王晓磊¹, 高炯¹, 周强², 徐永平², 蒯本科^1,*

¹复旦大学生命科学学院, 复旦大学植物研究所, 遗传工程国家重点实验室, 上海200433; ²上海华耘种业有限公司, 上海200335

通信作者：蒯本科；E-mail: bkkuai@fudan.edu.cn；Tel: 021-65642648

摘　要：

种子滞绿(stay-green)是豆科植物中的普遍现象, 最典型的例子就是孟德尔的绿粒豌豆。本文对十余份种子滞绿的大豆品种进行了初步分析, 发现滞绿性状表型上和遗传上呈现多样性特征。所分析的滞绿变异没有显著影响到叶片衰老进程中光合作用效率的下降, 但是明显影响衰老叶片中蛋白的降解与转运, 包括可溶性蛋白的降解与转运。我们对‘绿楂豆’等几个品种材料的滞绿性状的遗传和分子基础进行了初步的探索, 发现滞绿性状可能是受到两对遗传基因的控制, 很可能就是SGR1和SGR2。我们的初步分析结果提示, 大豆中受SGR1和SGR2共同调控的滞绿性状可能是源自于同一个早期变异事件。

关键词：大豆; 滞绿; 衰老; 光合作用效率; GmSGRs

收稿：2014-08-15   修定：2014-08-20

资助：复旦大学自主创新科技项目(培育)

A Preliminary Study on the Stay-Green Traits of Soybean Varieties

REN Jun¹, WANG Xiao-Lei¹, GAO Jiong¹, ZHOU Qiang², XU Yong-Ping², KUAI Ben-Ke^1,*

¹State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China; ^{2<1sup>Shanghai Huayun Seed Company, Shanghai 200335, China}

Corresponding author: KUAI Ben-Ke; E-mail: bkkuai@fudan.edu.cn; Tel: 021-65642648

Abstract:

Green seeds are widely observed in Leguminosae sp., as exemplified by classic Mendel’s green pea. In this study, we collected dozens of soybean varieties with green seeds and preliminarily analyzed the phenotypic, physiological, genetic and molecular aspects of their greenness traits. Their greenness was phenotypically classified into two general types: both cotyledons and seed coats showing greenness and only seed coat showing greenness. Those varieties possessing green cotyledons showed stay-green phenotypes during leaf senescence, and these stay-green traits were physiologically characterized as non-functional stay-green, i.e. net photosynthesis rates of senescent leaves declining as usual. However, both total and soluble proteins were significantly retained in their senescent leaves. We also preliminarily analyzed the inheritance of greenness traits in ‘Lvzhadou’ as well as in a few other varieties through their crosses with ‘William 82’, and found that they were principally regulated by two genetic loci. By PCR-based cloning techniques, we found that these greenness traits were associated with loss-of-function mutations in GmSGR genes across species. Along with a similar finding reported recently, our results imply that stay-green phenotypes caused by loss-of-function mutations in GmSGR genes are probably derived from the same mutation events.

Key words: soybean; stay-green; senescence; photosynthesis rate; GmSGRs